Expanded Synthetic Promoter Library Introduced to Boost Biomanufacturing Operations

SynGenSys, based in Sheffield, U.K., reports that it has introduced its CHO.SET^® 2.0 synthetic gene promoter system to improve protein production in CHO (Chinese Hamster Ovary) cells.

Developed de novo using the company’s proprietary transcriptional analysis and sequence engineering platform, CHO.SET synthetic promoters combine advanced computational design with empirical validation to deliver a promoter library applicable across a wide range of CHO expression systems and biomanufacturing requirements, according to Andy Racher, PhD, CEO, adding that new supporting data demonstrate that CHO.SET promoters deliver enhanced transcriptional activity and improved protein production metrics.

Selected vectors have been shown to achieve over 4x higher antibody productivity, with titers exceeding 2 g/L in a simple fed-batch flask model, he continues, while pointing out that the synthetic promoter pools also contain a higher proportion of high-expressing cells, increasing the likelihood of isolating top-performing clones.

“CHO cells are the pharmaceutical industry’s gold-standard cell factories, and CHO cell-specific synthetic promoters provide an elegant solution to overcoming key productivity limitations in the industry,” says Racher. “We are excited to release this latest update and to continue providing solutions that address evolving biomanufacturing needs.”

The CHO.SET Promoter Library will be displayed at BioProcess International next month as part of Biotech Week Boston.