October 15, 2018 (Vol. 38, No. 18)
The BlotCycler is a simple and elegant system designed to perform blocking, addition, and incubation of primary and secondary antibodies, and washing steps automatically. It is equipped with trays that can be configured to process up to 24 blots with 6 different primary and secondary antibodies simultaneously. Users simply add their antibodies and fill the BlotCycler tank with buffer solution, add blot membranes to processing trays, and select the protocol for blocking, incubation, and washing. With precise timing, the BlotCycler distributes buffers and antibodies and performs controlled agitation for consistent processing. When blots are removed, the automatic cleaning protocol is employed for thorough cleaning. The system accommodates most blocking, and incubation protocols and laboratories can continue using the same reagents. The BlotCycler is capable of running multiple Western blots using two different protocols simultaneously, performing all the steps in a precise, reproducible fashion to yield uniform assay results unattended.