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Transcriptomics studies are critical to understanding how biological systems work. However, this technique estimates protein function but is not a direct proteomic measurement, telling researchers what might occur in a cell if it neglects post-translational modifications and protein interactions.
While commonly used and more cost effective in recent years, traditional DNA and RNA sequencing alone do not tell the full story.
Proteomics provides more accurate picture of in vivo biology
In contrast, proteomics identifies the types and levels of proteins in the cell, and can help explain which proteins interact with each other.
Because proteins carry out cellular functions, proteomics is an effective tool for researching disease pathogenesis, developing therapeutics, and studying mechanisms of tumor resistance. For example, in cancer, researchers often look to genomic markers to understand signaling pathways, but functional adaptations can occur in the tumor that are unrecorded in genomic signatures: thus, it is important to look at intracellular proteomics.
Single-cell proteomics can provide a more complete picture of heterogenous cell populations, enabling identification of cellular subsets driving disease or response in a more predictive manner than single-cell transcriptomics alone.
Each immune cell is different; therefore, the complete characterization of each
individual cell is critical. While the transcriptome is measured via RNA sequencing, and surface phenotype is measured via flow cytometry, the complete cellular definition is incomplete without measuring extracellular cytokines that are doing the work in tumor immunology.
Uncover more meaningful data with IsoPlexis’ technology
IsoPlexis’ single-cell and highly multiplexed bulk secretomics provide sensitive immune profiling for a range of diseases and research areas, from infectious disease to cell therapy and cancer immunology.
We offer affordable single-cell and bulk solutions in a fully automatic, end-to-end solution to achieve a direct protein readout:
- Single-Cell Solutions: Our proprietary and patented IsoCode chips are at the core of our technology. With Single-Cell Secretome and Single-Cell Intracellular Proteome, you can analyze proteins both extra- and
intracellularly at the single-cell level, unlocking a higher level of resolution than conventional techniques. The Single-Cell chips work by detecting up to 32 proteins per cell, measuring the functional phenotype of each immune cell. This breakthrough technical innovation allows for complete single-cell functional characterization. - CodePlex Solution: A faster and more streamlined approach to generating multiplexed bulk cytokine data via a fully automated end-to-end workflow, requiring only five minutes of hands-on time. It does this by offering a modular solution to bulk cytokine data analysis and minimizing variability from user input.
- IsoSpeak Software: Our IsoSpeak software provides an application suite supporting each of our chips. This creates innovation, impactful end-to-end workflows, and applications for almost every key cell type, from early optimization to applying key, correlative insights and visualizations. Data is automatically analyzed for instant analysis and publication-ready figures.
To learn more about how IsoPlexis can help accelerate your therapeutics, visit our Single-Cell Proteomics Blog.