DNA Printing: Rapid Assembly of the RFP Gene with Enzymatic DNA Synthesis

DNA assembly is commonly performed in the build step of the design-build-test-learn cycle at the core of synthetic biology. In many assembly workflows, DNA is assembled into gene fragments, which are then assembled into larger constructs, often plasmids. Most research labs often generate gene fragments by restriction digest or PCR of existing templates, and in cases where new templates are needed, order them from external commercial suppliers.

During this event, we will learn about results from a recent study where gene fragments were assembled by polymerase cycling assembly from DNA oligos made in the lab using the SYNTAX System and enzymatic DNA synthesis (EDS). The synthesized gene fragments were then used immediately in a DNA assembly workflow to create a plasmid containing a functioning RFP (red fluorescent protein) gene. The ability to print DNA oligos on demand for assembly significantly shortened the time from design to build.

In this GEN webinar, our distinguished speaker, Kym Delventhal, will highlight the steps to successfully build a gene fragment for RFP gene synthesis from EDS oligos. We will then introduce the broad set of capabilities of EDS and the SYNTAX System and review data from applications including PCR, qPCR, NGS, Sanger sequencing, gene assembly, CRISPR, smFISH, and more.

A live Q&A session will follow the presentation, offering you a chance to pose questions to our expert panelist.

Produced with support from: