February 1, 2018 (Vol. 38, No. 3)
The NEBNext Ultra II FS DNA Library Prep Kit for next-generation sequencing (NGS) employs a novel enzymatic fragmentation system that combines fragmentation, end repair, and dA-tailing in a single tube, and utilizes a single protocol regardless of input amount or GC content. It has a streamlined workflow and library preparation can be completed within 2.5 hours, with less than 15 minutes of hands-on time. The high-efficiency Ultra II ligation reagents are provided for adaptor ligation, and while the kit is compatible with PCR-free workflows with inputs as low as 50 ng of eukaryotic DNA. The NEBNext Q5® Ultra II PCR Master Mix is provided for amplification of libraries when necessary. This kit is also available with the option of SPRISelect® beads for size-selection and clean-up steps.