Broadcast Date: May 5th, 2015
Time: 11:00 am ET, 8:00 am PT
Altered DNA methylation is an important regulator of gene expression contributing to a wide range of normal and disease phenotypes. While the number of whole genome bisulfite sequencing studies is rapidly growing, the sequencing costs associated with such studies can be quite large. Reduced Representation Bisulfite Sequencing (RRBS) allows researchers to obtain quantitative DNA methylation information across many important features of the genome with approximately 50-fold fewer sequencing reads than are needed with whole genome bisulfite sequencing, resulting in substantial sequencing cost reduction.
During this webinar we will describe and present data from a novel approach to RRBS. The Ovation® RRBS Methyl-Seq System can be used to generate RRBS libraries suitable for sequencing on Illumina platforms in a single day using 100 ng of human genomic DNA without gel-based size selection. The approach overcomes two challenges traditionally associated with the RRBS approach, namely the problem of low base diversity and the inability to identify PCR duplicates. Both problems have now been surmounted, allowing researchers to easily generate high-quality RRBS data.
Who Should Attend
- Cancer researchers
- R&D scientists
- Clinical research and development scientists
- Researchers interested in epigenetics
You Will Learn
- How RRBS can be used as a cost-effective method to survey samples for differential DNA methylation.
- How the Ovation RRBS Methyl-Seq System overcomes the problem of low base diversity and eliminates the need for PhiX controls.
- How the duplicate-marking capability used in the Ovation RRBS Methyl-Seq System enables an important data quality control, especially when working with limited or degraded samples.
Produced with support from:
Ben Schroeder, Ph.D.
Steven Kain, Ph.D.