The application, control, and removal of cell culture suspensions and experimental compounds are achieved by two open compound addition wells (O1 and O2), a switch valve channel (SW), and a waste well (W). Precise control over the pneumatic pressure applied to O1, SW, and O2 creates a tri-laminar flow with distinct cut-offs between each solution stream in the recording region microchannel.
During the early stages of recording, cells are loaded from well O1 and then control recording buffer perfused to obtain stable baseline recordings (Figure 1A and B). Application of vacuum to SW then disrupts the laminar streams for a time period that can be defined by the operator, and compound is applied from the O2 well (Figure 1C and D). The instrument is able to alter the compound concentration in O2 or exchange to a different compound allowing for multiple recordings from each recording group.