One of the most recent developments in the field of microwave peptide synthesis is in the synthesis of peptide nucleic acid (PNA) polyamides. PNA is a DNA mimic with an uncharged, pseudopeptide backbone. PNA oligomers form stable duplex structures with Watson-Crick complementary base pairing with DNA (or RNA) oligomers. PNAs also demonstrate high chemical and metabolic stability.
PNA oligomers have potential applications in antisense diagnostics and therapeutic areas. Fabani, Vigorito, and co-workers developed a method to synthesize PNA oligomers using microwave irradiation to accelerate the synthesis as well as to increase the yield and purity (Nucleic Acids Res., 2010).
Microwave irradiation has also been used to accelerate the synthesis of peptides containing sterically hindered amino acids, including N-methyl-rich peptides. N-methylated amino acid containing peptide analogues have improved pharmacological properties including enzyme stability, receptor selectivity, enhanced potency, and bioavailability. The coupling of these highly sterically hindered residues typically suffers from low yield and requires expensive coupling reagents.
Alberico et al. recently reported a method for the synthesis of N-methyl-rich peptides that utilizes microwave energy to accelerate the coupling time from two to four hours to only 20 minutes (J. Pept. Sci., 2010). They also performed the synthesis using the same time and temperature parameters under conventional conditions and reported that it resulted in low-purity peptides.
Microwave technology can also be used to promote the synthesis of cyclic peptides. Cyclic peptides are biologically interesting because they are typically resistant to digestion, a trait that makes them particularly suitable as peptide-based drugs. The head-to-tail cyclization of linear peptides in solution often suffers from oligomerization. In addition, the cyclization of tetrapeptides requires preorganization of the linear precursor.
Taddei developed a method using microwave irradiation to accelerate the cyclization, increase the yield, reduce the amount of solvent, and streamline the work-up and isolation procedure (Tetrahedron Lett., 2009).
Another area of microwave research that is receiving increasing attention is microwave-assisted proteomics and, more specifically, microwave-assisted enzymatic digestion of proteins for proteomic analysis. Higher efficiency digestion is obtained for trypsin in 15 minutes using microwaves compared to conventional overnight digestion at 37°C. This is reflected in higher database search score results, as well as higher intensity signals. The method has been applied successfully with solution and in-gel samples and is compatible with a range of enzymes including trypsin, Lys-C, and chymotrypsin.