Proteomics sample preparation refers to an assorted set of techniques, using various chemistries and devices, employed to extract or prefractionate protein samples prior to downstream applications for fractionation or analysis. Depending on a researcher’s specific aim or proteomics workflow, several procedures or methods are required for the appropriate preparation.
Techniques for proteomics sample preparation can be straightforward, such as the utilization of pipette tips packed with C18 silica for peptide microextraction prior to mass-spectrometry analysis. Methods can also be rather challenging, for example, the extraction and solubilization of membrane proteins prior to further fractionation by 2-D gel electrophoresis or chromatographic instrumentation.
Chemistries, devices, and kits for proteomics sample preparation are required in diverse life science research settings. Biopharmaceutical companies drive the greatest consumption of sample-preparation products and consequently are the strongest drivers of the market.
Biopharmaceutical firms, in their search for protein biomarkers or druggable protein targets, make use of high-throughput automated tools that allow for large-scale production. This necessitates a constant need for proteomics sample-preparation solutions.
Research initiatives, driven by the NIH or the Human Proteome Organization, also are fueling proteomics sample-preparation needs in academia. Scientists are increasingly interested in applying a proteomics-based analysis to their model systems.