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Spatial has emerged as a new frontier in omics analysis and is enabling the molecular and cellular state of tissues to be finely resolved. This is particularly important for cancer biology as the cellular arrangement of tumors impacts their growth, metastasis, and response to therapies, and thus identifying the genetic drivers of tumor architecture is a critical goal. While CRISPR screens are helping uncover genes regulating many cell-intrinsic processes, current approaches are limited in identifying gene functions that operate extracellularly or within a tissue context. Technologies are needed to bring CRISPR genomics into the spatial era.

In this GEN webinar, our distinguished speaker, Dr. Brian Brown, will discuss his approach for spatial functional genomics—dubbed Perturb-map—which employs protein barcodes (Pro-Code) to enable spatial detection of barcoded cells within tissue. Dr. Brown will describe how he and his colleagues applied Perturb-map to knockout dozens of genes in parallel within a mouse lung cancer model and simultaneously evaluated how each knockout influenced tumor growth, histopathology, and immune composition, as well as pairing Perturb-map with spatial transcriptomics for unbiased spatial analysis of CRISPR screens. Dr. Brown and his team see Perturb-map as providing a means to identify the genes controlling tumor architecture and casually link spatial omic findings with tumor biology.

 

A live Q&A followed the presentation, offering a chance to pose questions to our expert panelist.

Brian Brown, PhD
Brian Brown, PhD
Director, Icahn Genomics Institute
Associate Director, Precision Immunology Institute
Mount Sinai Medical Center