To fully characterize cellular function and how it pertains to patient outcomes such as antitumor response or disease progression, single-cell functional cytometry needs to play a critical role. The ability to perform deep functional multi-omics or complete single-cell proteomics—extracellular, intracellular, and metabolomics at single-cell and population resolution—using small sample sizes is paramount. In this GEN eBook, we will discover how single-cell analysis is shaping the way researchers approach cancer biology, and how a fully automated system called IsoLight is streamlining multi-omic and proteomic workflows for an array of precision medicine endeavors.