Dragana Catici
Dr. Dragana Catici
Lab Leader & Postdoctoral Researcher
Technical University of Munich
Nina Gubensak
Dr. Nina Gubensäk
Postdoctoral Research Fellow
University of Graz
Jan Schaefer
Dr. Jan Schaefer
Field Application Scientist

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Microfluidic Modulation Spectroscopy (MMS) is a novel QCL-based Infrared-spectroscopic technique that generates ultra-sensitive and precise structural measurements of proteins and other biomolecules with greater sensitivity than traditional spectroscopic tools. 

This webinar, led by Dr. Dragana Catici of the Technical University of Munich and Dr. Nina Gubensäk of the University of Graz, provides examples of how MMS was used with minimal time expenditure to generate data that helped elucidate protein structure-function relationships in each researcher’s area of interest.

Dr. Catici will explore the effect of high protein concentration (macromolecular crowding) on the structure, stability, and function of alpha-crystallin proteins. MMS was used to examine structural changes upon protein crowding, over a very broad concentration range. At the lower end of the concentration range, MMS was compared to CD, with results showing that MMS provides a more realistic picture of the absolute protein structure and that MMS is able to identify the subtle structural changes associated with crowding effects.

Dr. Gubensäk will offer an insight into the regulatory bile-sensing protein complex, ToxRS of V. cholerae. The analysis included the application of MMS, which was used to identify structural changes upon ligand binding, which initiates virus activation. These findings will aid future development of therapeutics to disrupt virulent action.

The presentation will conclude with a brief overview of the fundamentals of MMS.

Join us as we explore these two data sets in greater detail and learn about the key elements of MMS that differentiate it from classic protein secondary structure tools, and which helped deliver high-impact results for these researchers as well as numerous others in both academia and biopharmaceutical development.

A live Q&A session followed the presentation, offering a chance to pose questions to our expert panelists.

Produced with support from:

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