Broadcast Date: 
  • Time: 

Oligosaccharide profiling of biopharmaceutical and other glycoprotein products remains challenging for scientists who report that regardless of the technique used, more than one technique is often required for complete oligosaccharide identification. Recent collaborative studies have noted that while MALDITOF MS was faster, High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD), provided significantly better precision, allowing identification of structural isomers and simultaneous analysis of neutral and sialylated oligosaccharides.

Described for separations of asparagine-linked (N-linked) glycoproteins in 1988, HPAE-PAD has become a key technique for characterizing recombinant therapeutic glycosylation because it can be used for monosaccharide, sialic acid, and oligosaccharide analyses (N-linked and Ser/Thr-linked [O-linked] oligosaccharides. HPAE-PAD can determine these analytes without derivatization while providing high selectivity and sensitivity in various samples matrices.

HPAE-PAD is particularly valuable for oligosaccharide analysis with the value derived from the high-resolution separation followed by sensitive detection of native oligosaccharides. In this presentation the application of HPAE-PAD to oligosaccharides released from glycoproteins, will be discussed.

Who Should Attend

  • Biochemists
  • Biopharmaceutical developers
  • Scientists developing biopharmaceutical analytical workflows
  • Scientists developing methods to profile the oligosaccharides from glycoprotein biopharmaceuticals to ensure product consistency

You Will Learn

  • How the fundamentals of HPAE-PAD are used to achieve high-resolution oligosaccharide analysis
  • How HPAE-PAD is applied to profiling oligosaccharides from glycoproteins
  • How HPAE-PAD complements other oligosaccharide analysis techniques

Produced with support from:

Sponsor Logo


Jeffrey Rohrer, Ph.D.,
Director of Applications Development,
Thermo Fisher Scientific