Broadcast Date: March 25th, 2015
Time: 11:00 am ET, 8:00 am PT

In comparison to other biomolecules, and despite their significance in biopharmaceuticals, glycans have been historically problematic to manipulate and study. Advances in technology for glycan analysis has lagged behind and been a hindrance for many researchers.

Furthermore, conventional sample-preparation workflows for released glycan analysis are notoriously laborious, time-consuming, and usually lead to compromises in sensitivity. This limits both the throughput and detail by which the glycosylation of a biopharmaceutical can be profiled.

New reagent chemistries for N-glycan labeling promise to not only modernize the sample-preparation workflow for released N-glycans, but also make mass spectrometry readily accessible by improving glycan monitoring and characterization.

In this GEN webinar, Matthew Lauber, Ph.D., senior applications chemist for Waters, will present a streamlined glycan sample-preparation workflow utilizing the new GlycoWorks™ RapiFluor-MS™ N-Glycan Kit, which allows researchers to rapidly deglycosylate, label, and clean-up samples for LC/FLR analysis in three easy steps within 30 minutes.

Dr. Lauber will describe in detail the labeling reagent RapiFluor-MS, which delivers enhanced ionization capabilities, allowing glycans to be detected using the ACQUITY® QDa® Detector. With the high sensitivity enabled by this new reagent, scientists will be able to increase throughput and have access to mass detection as an orthogonal technique to confirm FLR peaks in glycan monitoring.

A live Q&A session will follow the presentation, offering you a chance to pose questions to our expert panelist.

Who Should Attend

  • Biochemists
  • Biopharmaceutical and therapeutic protein developers
  • Scientists developing biopharmaceutical analytical workflows
  • QC/QA scientists manufacturing therapeutic proteins
  • Protein analytical scientists interested in glycan analysis

You Will Learn

  • Details of the three streamlined steps that provide complete sample preparation within 30 minutes for most samples:
    • Rapid deglycosylation that works for a variety of simple to complex glycoproteins.
    • Fast labeling chemistry that provides increased levels of sensitivity for both fluorescence and mass spectral response making assignment of low abundance glycans easier.
    • Robust sample cleanup method that provides high recovery up to tetrasialyated glycans using animopropyl, HILIC μElution and a custom elution buffer.
  • How to complement FLR-based glycan monitoring with mass data for peak confirmation.

Produced with support from:

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Panelists

Matthew A. Lauber, Ph.D.
Principal Applications Chemist,
Waters Corporation