February 15, 2018 (Vol. 38, No. 4)
ZEISS
The LSM 8 family with Airyscan features an area detector, and unlike traditional confocal microscopes (which reject photons from outside of the focal plane at a pinhole), the Airyscan detects all precious fluorescence emission photons of 1.25 AU. Their information is then used to deliver higher sensitivity, super-resolution, and high acquisition speeds. The new 2D Superresolution mode takes advantage of the fact that Airyscan captures x, y, and z information of the confocal point spread function. A new processing algorithm uses this inherent spatial information captured in a single image. It specifically distinguishes between photons originating from the focal plane of 0.2 AU and photons from outside of this focal plane.
