This new series of Zika virus assays have little or no cross-reactivity with other flaviviruses. The Zika Virus NS1 ELISA assay is a highly sensitive and specific assay suitable for the quantitative detection of Zika Virus NS1 in a range of biological samples. This ELISA assay can detect NS1 antigen at levels as low as 5 pg/mL and enables researchers to measure the level of Zika NS1 protein in infected patients, offering value in epidemiology studies. The Zika Virus IgG/IgM/IgA ELISA assay is designed for the detection of Zika-specific antibodies in human serum. It is minimally cross-reactive with antibodies to Dengue virus and so can be used to distinguish human anti-Zika antibodies from other flavivirus and infectious disease antibodies. This assay has a short assay time of only two hours, with specificity and sensitivity both in excess of 90%, even in regions endemic with Dengue. Due to the novel assay format, the test may also be used in any species, making it of value for studying animal models of Zika infection.