February 1, 2018 (Vol. 38, No. 3)
This single nucleus RNA-seq chip is designed to produce smaller droplets, allowing straightforward, low-cost profiling of thousands of nuclei. Nuclei can be quickly and easily isolated from frozen or lightly fixed tissues using DronNc-seq. This avoids lengthy and stressful manipulation of live cells, which could perturb expression profiles. The transcriptomes acquired are very similar to those from whole cells, albeit with a lower total transcript capture rate and greater representation of nuclear RNA and pre-mRNA. The single nucleus RNA-seq chip is ideal for nuclei encapsulation and has been optimized for the generation of smaller diameter droplets than those used in standard scRNA-seq protocols.