October 15, 2017 (Vol. 37, No. 18)
A new chip for the DroNc-seq enables high-throughput single-nucleus RNA-seq using droplet microfluidics. The single nucleus RNA-seq chip is designed to produce smaller droplets, allowing straightforward, low-cost profiling of thousands of nuclei. DroNc-seq is a novel, single-cell RNA-seq methodology, which uses isolated nuclei instead of whole cells. Nuclei can be quickly and easily isolated from frozen or lightly fixed tissues—including tissues from which individuals cells are difficult to isolate. The single nucleus RNA-seq chip is ideal for nuclei encapsulation and has been optimized for the generation of smaller diameter droplets than those used in standard scRNA-seq protocols.