Biological samples commonly contain proteins that interfere with downstream applications such as liquid chromatography-mass spectrometry (LC-MS) analysis. If proteins are not removed, they can precipitate out into the high-performance liquid chromatography (HPLC) mobile phase leading to column or system blockages. The Microlute™ Protein Precipitation Plate (PPP) has been designed to help labs looking to remove interfering proteins from serum, plasma, or even whole blood samples prior to analysis by LC-MS. The Microlute PPP uses the CRASH method, in which protein is denatured with acetonitrile and the flocculant filtered out, allowing 96 samples to be processed in parallel. The protein “crashes” out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated with the CRASH technique of protein cleanup. According to the company, the novel dual frit eliminates the “wetting out” and leakage of sample before the application of vacuum.
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