The P3 microplate works by denaturing the protein upon addition of solvents, such as acetonitrile or methanol. The proteins precipitate out of solution to form large sticky globules that are then trapped on an open-pore structure filter. Underneath this filter is a highly hydrophobic filter which retains all liquids above it. When a vacuum is applied to the P3 microplate, the liquid containing the analytes of interest is efficiently drawn through the filter and into a collection plate below, leaving behind unwanted proteins that can interfere with downstream applications. The novel dual filter technology in the P3 microplate not only retains unwanted proteins and has good flow rates, it can also ensure no leakage occurs prior to collection by vacuum. Designed and precision manufactured to conform with ANSI/SLAS guidelines, the automation-friendly P3 microplate enables 96 samples to be filtered simultaneously.