September 15, 2018 (Vol. 38, No. 16)
StarBright Blue 520 Fluorescent Secondary Antibodies are fluorescent dye-labeled secondary antibodies for use in multiplex Western blotting. They exhibit a two- to threefold lower limit of detection than traditional green emission fluorophore-labeled antibodies. The StarBright Blue line of secondary antibodies enables highly sensitive fluorescent detection and short exposure times. These plug-and-play antibodies allow simultaneous detection of up to three proteins (two targets of interest and one housekeeping protein) on the same blot when used with the hFAB rhodamine housekeeping protein fluorescent primary antibodies. The antibodies work seamlessly on nitrocellulose or low-fluorescence PVDF membranes, and all other aspects of the standard Western blotting workflow remain unchanged. They are labeled with a particularly bright fluorescent dye, resulting in short exposure times and a high signal-to-noise ratio.