The BioCool V40 is a mechanically refrigerated 1.5-L system designed for controlled rate freezing of biological materials including blood, embryos, and tissue samples. The BioCool V40 is the only controlled rate freezer that does not require expendable liquid nitrogen and the associated pumping, refilling, and storage challenges posed by a cryogenic liquid. The system plugs into a standard electrical outlet and quietly provides low temperature cooling to −40°C. The magnetic stirrer and vortex breaker of the BioCool V40 ensure isothermal conditions throughout its temperature range. An intuitive user interface allows for easy navigation and parameter adjustment. The controller enables programming and storage of numerous protocols, each with up to 10 distinct segments specifying ramp rate, hold temperature, and hold time.