The P3 microplate can be used to accelerate the removal of interfering proteins from serum, plasma, or even whole blood samples prior to analysis by LC–MS. Biological samples commonly contain proteins that interfere with downstream applications. Traditionally, scientists have used the CRASH method, in which the protein is denatured with acetonitrile and the flocculant filtered out to clean their samples prior to LC–MS analysis. Using a P3 protein “crashes” out of solution and precipitates directly in each well of the microplate when acetonitrile is added, thus solving all common problems traditionally associated with the CRASH technique of protein clean-up. The novel dual frit, hydrophobically treated matrix used by the P3 microplate means that there is no “wetting out” and leakage of the sample before the application of vacuum. The P3 microplate enables 96 samples to be filtered simultaneously—accelerating LC–MS protocols.