May 15, 2018 (Vol. 38, No. 10)
The lncRNA Workflow is an RT-qPCR workflow that is optimized for highly sensitive and specific quantification of long noncoding RNAs (lncRNAs) for gene expression analysis. The workflow provides a streamlined, cost-effective alternative to RNA-seq for lncRNA discovery and validation. Quantification of lncRNAs is particularly difficult due to low expression levels seen in some developmental contexts or with certain cell types. Furthermore, lncRNA primer design requires considerable time and effort as targets are often positioned in complex genomic loci and can form secondary structures. PrimePCR lncRNA assay designs were developed in collaboration with transcriptome experts at BioGazelle using a design algorithm specifically built for lncRNAs. The lncRNA Workflow solves the most pressing challenges in lncRNA discovery and validation with its workflow that includes PrimePCR lncRNA Assays, Predesigned PrimePCR lncRNA Arrays, Custom PrimePCR lncRNA Arrays, and the novel iScript Explore One-Step RT and PreAmp Kit. The workflow offers all the tools necessary to go from experimental sample to lncRNA data analysis.