September 1, 2017 (Vol. 37, No. 15)
The MACSprep PBMC Isolation Kit can be used for the straightforward isolation of PBMCs from 2–10 mL of human whole blood without the requirement of density-gradient centrifugation. This kit offers fast and easy PBMC preparation in only 25 minutes, and the isolation can be easily performed in a two-step separation procedure, resulting in highly pure PBMCs ready to be used for downstream experiments. Fields of application include: PBMC co-cultures for functional immune cell assays, studies on cytokine expression of immune cells upon restimulation, studies on signal transduction during activation of immune cells, and immune monitoring of antigen-specific T cells.