The StemFit® for differentiation is a new chemically defined and animal component-freeformulation that enables differentiation of human Induced Pluripotent Stem (hiPS) and Embryonic Stem (hES) cells.The composition of StemFit for Differentiation minimizes lot-to-lot variation, enabling consistent cell differentiation. Free of animal- and human-derived components, StemFit® for differentiation can be used to eliminate the risk of immunogenic contamination. Applications that can be used include lineage-specific (endodermal, mesodermal, and ectodermal) differentiation, where this new product is used to replace serum-free supplements, as well as spontaneous differentiation of hiPSCs to organoids via embryoid body formation.