February 15, 2018 (Vol. 38, No. 4)
This custom, synthetic mRNA service uses Ψ-containing mRNA, which is translated more efficiently than its unmodified counterpart, resulting in higher protein expression and reduction of the innate antiviral response. The service includes synthesis of DNA; plasmid cloning; purification; full DNA sequencing documentation; PCR amplification of the cloned insert; purification and subsequent RNA synthesis by in vitro transcription; purification; and characterization. This synthetic mRNA is made to research grade for development applications, and GMP grade for cell therapy.