March 1, 2018 (Vol. 38, No. 5)
Sequencing cell-free DNA (cfDNA) fragments, a biomarker used to detect and treat cancer via a liquid biopsy, needs an optimal DNA concentration during flow-cell loading. These high-sensitivity fragment analysis kits can measure the size and concentration of the three common cfDNA fragments—the ~165 bp mono-nucleosome, the ~350 bp di-nucleosome, and the ~565 bp tri-nucleosome—using only 2 mL of sample. The kits use internal standards, which enables accurate DNA quantification within 25%. The sizing accuracy for their small fragment and NGS kits is within 5%, while the accuracy of the large fragment kit is around 15%. Runs are completed in less than an hour. Each kit contains all materials needed for an optimized capillary electrophoresis experiment on cfDNA.