Pico-Surf™ has been designed for reliable and highly stable droplet generation and processing. Pico-Surf is described as a high-quality and animal-origin-free biocompatible surfactant optimized to support the formation of aqueous solution-in-oil picodroplets. Pico-Surf is designed to work effectively and flexibly across a broad range of microfluidic systems. The molecular structure of Pico-Surf stabilizes droplets and retains and protects their cellular and molecular contents over a wider range of temperatures and biological conditions, helping to ensure high cell viability for improved assay performance.