November 15, 2017 (Vol. 37, No. 20)
The Contichrom Discovery is an automated FPLC system for the purification of tagged proteins and antibodies. It combines two functional features: full single-column and automated multistep batch purification. Up to three connected chromatography steps can be run in one process without user intervention. For example, antibodies can be purified with one process including Protein A affinity, cation-exchange polishing, and desalting steps. Up to 19 three-step purification runs can be performed per day. The system has an inbound fraction collector for four fractions. An external fraction collector is available optionally. With a hardware and software upgrade, the system can be converted into a high-throughput model with the capability for unattended purification of up to 18 consecutive samples per sequence.