Home Topics Drug Discovery Protein and RNA Used to Create Vesicles for Potential Drug Delivery

Protein and RNA Used to Create Vesicles for Potential Drug Delivery

Protein-RNA vesicles (fluorescence microscopy)
A fluorescence microscopy image shows protein-RNA vesicles created in the lab. The vesicles -- hollow, spherical sacks -- were made without traditional lipid building blocks. [Ibraheem Alshareedah]

Using protein and RNA, scientists say they have created hollow, spherical sacks, i.e., vesicles, which hold potential as biological storage compartments. They could serve as an alternative to traditional vesicles that are made from water-insoluble lipids, the researchers say.

“Our discovery has widespread implications, from understanding basic cell biology to enabling possible biotechnological applications, such as targeted drug delivery or pesticide release,” says Priya Banerjee, PhD, assistant professor of physics in the University at Buffalo College of Arts and Sciences. “These hollow spheres look and behave like the classical lipid vesicles that people have been utilizing in bioengineering applications for many years, with an important exception: They are not made by lipids.”

“Because of the hollow structure resembling lipid vesicles, one may envision a potentially exciting application where protein-RNA vesicles are dispatched into cells for carrying out rescue missions by releasing biomolecules or changing the local subcellular environment,” adds Davit Potoyan, PhD, assistant professor of chemistry at Iowa State University. “Another reason to be excited is that these vesicles are formed spontaneously from naturally occurring proteins and nucleic acids, which may help to avoid issues of toxicity that might be seen in other polymers designed to mimic lipid vesicles.”

The study “Phase transition of RNA−protein complexes into ordered hollow condensates” appears in PNAS.

“Liquid−liquid phase separation of multivalent intrinsically disordered protein−RNA complexes is ubiquitous in both natural and biomimetic systems. So far, isotropic liquid droplets are the most commonly observed topology of RNA−protein condensates in experiments and simulations. Here, by systematically studying the phase behavior of RNA−protein complexes across varied mixture compositions, we report a hollow vesicle-like condensate phase of nucleoprotein assemblies that is distinct from RNA−protein droplets,” write the investigators.

“We show that these vesicular condensates are stable at specific mixture compositions and concentration regimes within the phase diagram and are formed through the phase separation of anisotropic protein−RNA complexes. Similar to membranes composed of amphiphilic lipids, these nucleoprotein−RNA vesicular membranes exhibit local ordering, size-dependent permeability, and selective encapsulation capacity without sacrificing their dynamic formation and dissolution in response to physicochemical stimuli.”

“Our findings suggest that protein−RNA complexes can robustly create lipid-free vesicle-like enclosures by phase separation.”

Protein-RNA vesicles (polarization microscopy)
A polarization microscopy image of protein-RNA vesicles created in the lab. The vesicles — hollow, spherical sacks — were made without traditional lipid building blocks. The microscopy image shows an optical birefringent pattern typical of liquid crystalline materials. [Ibraheem Alshareedah]
Banerjee and Potoyan led the project, along with Ibraheem Alshareedah, a UB physics PhD student, and Mahdi Muhammad Moosa, PhD, a UB physics postdoctoral scholar, who did the experiments; and Muralikrishna Raju, PhD, an Iowa State chemistry postdoctoral scholar who carried out computer simulations.

To make the micron-sized vesicles, scientists mixed naturally occurring cationic proteins with RNA in an aqueous buffer solution. At some concentrations, the protein and RNA molecules clustered together to produce liquid droplets, akin to beads of oil floating in water. But at other concentrations, the protein and RNA instead came together to form the bubble-like vesicles.

As part of the research, the team also mapped out the conditions under which each type of structure forms. Experiments and simulations showed that the protein-RNA complexes coalesce spontaneously and are held together because of weak electrostatic attraction, repulsive interactions and chain entropy. A delicate balance of these forces dictates whether liquid droplets or hollow vesicles will form, the researchers say.

Protein-RNA vesicles (differential interference contrast microscopy)
Differential interference contrast microscopy images show protein-RNA vesicles created in the lab. The vesicles — hollow, spherical sacks — were made without traditional lipid building blocks. The scale bar shows a length of 10 microns. [Ibraheem Alshareedah]
“We argue that there is a tradeoff between these forces,” notes Banerjee. “When you have too much repulsion, the protein and RNA molecules stay separated. But when you balance these intricate forces, you see a stabilization of these different structures, liquid droplets or hollow condensates.”

After discovering the principle of how the vesicles are formed, the team created similar hollow droplets using other building blocks, such as designed polypeptides and synthetic polymers, demonstrating that the findings could have broad applications.

“We also observed that similar lipid-free vesicles can form with many proteins and RNA mimetics,” Moosa points out. “The ability to pick and choose from a large pool of mimetics will allow customizable biotechnological application of these assemblies.”

UB has filed a provisional patent application for the lipid-free protein-RNA vesicles and the methods for making the vesicles.

The new study resulted from Banerjee’s past work on protein-RNA complexes, which focused on generating liquid droplets from protein and RNA molecules.

In one experiment, “When we added more RNA to the droplets, immediately these compartments, these beautiful bubble-like structures, started forming inside those droplets,” Banerjee recalls. “But it wasn’t stable, and it went away in a few minutes.”

That curious observation led his team to start investigating how to create the hollow structures and keep them intact for longer periods, which the new study achieves. The research on both liquid and hollow droplets could lead to improved understanding of how similar compartments form spontaneously inside human cells, according to Banerjee.

One intriguing aspect of the study is the formation of vesicular geometry, reminiscent of membranes, arising from protein and RNA molecules, the scientists say. Lipids are well-known building blocks for biological membranes, but the new study suggests that other possibilities may exist for creating membrane-like barriers in biological systems that were previously not known, Banerjee says.

Future studies could explore whether and how cells might use protein-RNA membranes to perform certain biological functions.