For a long time, people have been interested in adapting bioadhesive materials to replace sutures and staples for sealing tissues during minimally invasive surgical procedures. There are many surgeries where a small incision is made and miniature cameras and surgical tools are threaded through the body to remove tumors and repair damaged tissues and organs. While many procedures can be performed in this way, surgeons can face challenges at an important step in the process—the sealing of internal wounds and tears.

Now, MIT engineers have developed a new strategy for minimally invasive tissue sealing. Taking inspiration from origami, they have designed a medical patch that can be folded around minimally invasive surgical tools and delivered through airways, intestines, and other narrow spaces, to patch up internal injuries. The patch resembles a foldable, paper-like film when dry. Once it makes contact with wet tissues or organs, it transforms into a stretchy gel, similar to a contact lens, and can stick to an injured site.

In contrast to existing surgical adhesives, the team’s new tape is designed to resist contamination when exposed to bacteria and bodily fluids. Over time, the patch can safely biodegrade away.

The researchers are working with clinicians and surgeons to optimize the design for surgical use, and they envision that the new bioadhesive could be delivered via minimally invasive surgical tools, operated by a surgeon either directly or remotely via a medical robot.

The work is published in Advanced Materials in the paper, “A Multifunctional Origami Patch for Minimally Invasive Tissue Sealing.

“Minimally invasive surgery and robotic surgery are being increasingly adopted, as they decrease trauma and hasten recovery related to open surgery. However, the sealing of internal wounds is challenging in these surgeries,” said Xuanhe Zhao, PhD, professor of mechanical engineering and of civil and environmental engineering at MIT.

“This patch technology spans many fields,” added co-author Christoph Nabzdyk, MD, a cardiac anesthesiologist and critical care physician at the Mayo Clinic in Rochester, Minnesota. “This could be used to repair a perforation from a coloscopy, or seal solid organs or blood vessels after a trauma or elective surgical intervention. Instead of having to carry out a full open surgical approach, one could go from the inside to deliver a patch to seal a wound at least temporarily and maybe even long-term.”

The bioadhesives currently used in minimally invasive surgeries are available mostly as biodegradable liquids and glues that can be spread over damaged tissues. When these glues solidify, however, they can stiffen over the softer underlying surface, creating an imperfect seal. Blood and other biological fluids can also contaminate glues, preventing successful adhesion to the injured site. Glues can also wash away before an injury has fully healed, and, after application, they can also cause inflammation and scar tissue formation.

Given the limitations of current designs, the team aimed to engineer an alternative that would meet three functional requirements. It should be able to stick to the wet surface of an injured site, avoid binding to anything before reaching its destination, and once applied to an injured site resist bacterial contamination and excessive inflammation.

The team’s design meets all three requirements, in the form of a three-layered patch. The middle layer is the main bioadhesive, made from a hydrogel material that is embedded with compounds called NHS esters. When in contact with a wet surface, the adhesive absorbs any surrounding water and becomes pliable and stretchy, molding to a tissue’s contours. Simultaneously, the esters in the adhesive form strong covalent bonds with compounds on the tissue surface, creating a tight seal between the two materials. The design of this middle layer is based on previous work in Zhao’s group.

The team then sandwiched the adhesive with two layers, each with a different protective effect. The bottom layer is made from a material coated with silicone oil, which acts to temporarily lubricate the adhesive, preventing it from sticking to other surfaces as it travels through the body. When the adhesive reaches its destination and is pressed lightly against an injured tissue, the silicone oil is squeezed out, allowing the adhesive to bind to the tissue.

The adhesive’s top layer consists of an elastomer film embedded with zwitterionic polymers, or molecular chains made from both positive and negative ions that act to attract any surrounding water molecules to the elastomer’s surface. In this way, the adhesive’s outward-facing layer forms a water-based skin, or barrier against bacteria and other contaminants.

“In minimally invasive surgery, you don’t have the luxury of easily accessing a site to apply an adhesive,” said Hyunwoo Yuk, a graduate student in the Zhao lab. “You really are battling a lot of random contaminants and body fluids on your way to your destination.”

In a series of demonstrations, the researchers showed that the new bioadhesive strongly adheres to animal tissue samples, even after being submerged in beakers of fluid, including blood, for long periods of time.

They also used origami-inspired techniques to fold the adhesive around instruments commonly used in minimally invasive surgeries, such as a balloon catheter and a surgical stapler. They threaded these tools through animal models of major airways and vessels, including the trachea, esophagus, aorta, and intestines. By inflating the balloon catheter or applying light pressure to the stapler, they were able to stick the patch onto torn tissues and organs, and found no signs of contamination on or near the patched-up site up to one month after its application.

The researchers envision that the new bioadhesive could be manufactured in prefolded configurations that surgeons can easily fit around minimally invasive instruments as well as on tools that are currently being used in robotic surgery. They are seeking to collaborate with designers to integrate the bioadhesive into robotic surgery platforms.

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