This video provides step-by-step user instructions for two-dimensional (2D) protein electrophoresis.

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Are you perplexed by all the steps involved in running a 2D electrophoresis gel? Fear not! GEN has the guide you’ve been looking for. In this video, Steve Freeby of Bio-Rad Laboratories provides complete, step-by-step user instructions for two-dimensional (2D) protein electrophoresis using IPG strips and polyacrylamide gels. Topics covered include how to apply samples and rehydrate the IPG strips, how to equilibrate the IPG strips prior to running on the second-dimension gel, and staining and visualizing proteins on the finished gel. Practical tips are given at each step to ensure high-quality results using Bio-Rad equipment and reagents.

Click here to watch the video.

For even more helpful information on how to run your gels, don’t miss GEN’s 20 Tips for Terrific 2D Gel Electrophoresis Results!

To learn more about Bio-Rad and its life science products, go to

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