I know what you’re thinking, did I not have enough coffee this morning and totally misread that title? Well, don’t fret. Your groggy eyes did not deceive you, as investigators at the Monell Chemical Senses Center in Philadelphia have made an intriguing discovery, which shows that functional olfactory receptors, the sensors that detect odors in the nose, are also present in human taste cells found on the tongue. Findings from the new study—published today in Chemical Senses through an article titled “Mammalian Taste Cells Express Functional Olfactory Receptors”—suggest that interactions between the senses of smell and taste, the primary components of food flavor, may begin on the tongue and not in the brain, as previously thought. The identification of functional olfactory receptors in human taste cells opens doors to new approaches to modify food flavor, which could help cut overall food intake, reducing metabolic disorders.
“Our research may help explain how odor molecules modulate taste perception,” explains senior study investigator Mehmet Hakan Ozdener, MD, PhD, a cell biologist at Monell. “This may lead to the development of odor-based taste modifiers that can help combat the excess salt, sugar, and fat intake associated with diet-related diseases such as obesity and diabetes.”
Most people equate flavor with taste, the distinctive flavor of most foods and drinks comes more from smell than it does from taste. Taste, which detects sweet, salty, sour, bitter, and umami (savory) molecules on the tongue, evolved as a gatekeeper to evaluate the nutrient value and potential toxicity of what we put in our mouths. Smell provides detailed information about the quality of food flavor, for example, is that banana, licorice, or cherry? The brain combines input from taste, smell, and other senses to create the multi-modal sensation of flavor.
Yet until now, taste and smell were considered to be independent sensory systems that did not interact until their respective information reached the brain. Ozdener was prompted to challenge this belief when his 12-year-old son asked him if snakes extend their tongues so they can smell.
In the current study, the research team used methods developed at Monell to maintain living human taste cells in culture. Using genetic and biochemical methods to probe the taste cell cultures, the researchers found that the human taste cells contain many key molecules known to be present in olfactory receptors. The team subsequently used calcium imaging to show that the cultured taste cells respond to odor molecules in a manner similar to olfactory receptor cells.
“We report that olfactory receptors are functionally expressed in taste papillae,” the authors write. “We found expression of olfactory receptors in the taste papillae of green fluorescent protein-expressing transgenic mice and, using immunocytochemistry and real-time quantitative polymerase chain reaction experiments, the presence of olfactory signal transduction molecules and olfactory receptors in cultured human fungiform taste papilla (HBO) cells. Both HBO cells and mouse taste papilla cells responded to odorants. Knockdown of adenylyl cyclase mRNA by specific small inhibitory RNA and pharmacological block of adenylyl cyclase eliminated these responses, leading us to hypothesize that the gustatory system may receive olfactory information in the periphery.”
These findings provide the first demonstration of functional olfactory receptors in human taste cells, suggesting that olfactory receptors may play a role in the taste system by interacting with taste receptor cells on the tongue. Supporting this possibility, other experiments by the Monell scientists demonstrated that a single taste cell can contain both taste and olfactory receptors.
“The presence of olfactory receptors and taste receptors in the same cell will provide us with exciting opportunities to study interactions between odor and taste stimuli on the tongue,” said Ozdener.
In addition to providing insight into the nature and mechanisms of smell and taste interactions, the findings also may provide a tool to increase understanding of how the olfactory system detects odors. Scientists still do not know what molecules activate the vast majority of the 400 different types of functional human olfactory receptors. Because the cultured taste cells respond to odors, they potentially could be used as screening assays to help identify which molecules bind to specific human olfactory receptors.
Looking toward the future, the scientists will seek to determine whether olfactory receptors are preferentially located on a specific taste cell type, for example, sweet- or salt-detecting cells. Other studies will explore how odor molecules modify taste cell responses and, ultimately, human taste perception.
