Scientists at MIT and the Catholic University of Brasilia say they have come up with a streamlined approach to developing antimicrobial peptides as potential drugs to fight antibiotic-resistant bacteria. Their novel strategy, which relies on a computer algorithm that mimics the natural process of evolution, has already yielded one potential drug candidate that successfully killed bacteria in mice.

“We can use computers to do a lot of the work for us, as a discovery tool of new antimicrobial peptide sequences,” says César de la Fuente-Núñez, Ph.D., an MIT postdoc and Areces Foundation Fellow. “This computational approach is much more cost-effective and much more time-effective.”

Dr. de la Fuente-Núñez and Octavio Franco, Ph.D., of the Catholic University of Brasilia and the Dom Bosco Catholic University, are the corresponding authors of the paper (“In Slico Optimization of a Guava Antimicrobial Peptide Enables Combinatorial Exploration for Peptide Design”), which appears in Nature Communications. Timothy Lu, Ph.D., an MIT associate professor of electrical engineering and computer science, and of biological engineering, is also an author.

“Plants are extensively used in traditional medicine, and several plant antimicrobial peptides have been described as potential alternatives to conventional antibiotics. However, after more than four decades of research no plant antimicrobial peptide is currently used for treating bacterial infections, due to their length, post-translational modifications or high dose requirement for a therapeutic effect. Here we report the design of antimicrobial peptides derived from a guava glycine-rich peptide using a genetic algorithm. This approach yields guavanin peptides, arginine-rich α-helical peptides that possess an unusual hydrophobic counterpart mainly composed of tyrosine residues,” write the investigators.

“Guavanin 2 is characterized as a prototype peptide in terms of structure and activity. Nuclear magnetic resonance analysis indicates that the peptide adopts an α-helical structure in hydrophobic environments. Guavanin 2 is bactericidal at low concentrations, causing membrane disruption and triggering hyperpolarization. This computational approach for the exploration of natural products could be used to design effective peptide antibiotics.”

In their search for more powerful, artificial antimicrobial peptides, scientists typically synthesize hundreds of new variants, which is a laborious and time-consuming process, and then test them against different types of bacteria. Dr. de la Fuente-Núñez and his colleagues wanted to find a way to make computers do most of the design work. To achieve that, the researchers created a computer algorithm that incorporates the same principles as Darwin's theory of natural selection. The algorithm can start with any peptide sequence, generate thousands of variants, and test them for the desired traits that the researchers have specified. 

“By using this approach, we were able to explore many, many more peptides than if we had done this manually. Then we only had to screen a tiny fraction of the entirety of the sequences that the computer was able to browse through,” Dr. de la Fuente-Núñez says. 

In this study, the researchers began with an antimicrobial peptide found in the seeds of the guava plant. This peptide, known as Pg-AMP1, has only weak antimicrobial activity. The researchers told the algorithm to come up with peptide sequences with two features that help peptides to penetrate bacterial membranes—a tendency to form α-helices and a certain level of hydrophobicity. 

After the algorithm generated and evaluated tens of thousands of peptide sequences, the researchers synthesized the most promising 100 candidates to test against bacteria grown in lab dishes. The top performer, guavanin 2, contains 20 amino acids. Unlike the original Pg-AMP1 peptide, which is rich in the amino acid glycine, guavanin is rich in arginine but has only one glycine molecule. 

These differences make guavanin 2 much more potent, especially against Gram-negative bacteria, which include many species responsible for the most common hospital-acquired infections, including pneumonia and urinary tract infections. 

The researchers tested guavanin 2 in mice with a skin infection caused by Pseudomonas aeruginosa, and found that it cleared the infections much more effectively than the original Pg-AMP1 peptide. 

“This work is important because new types of antibiotics are needed to overcome the growing problem of antibiotic resistance,” says Mikhail Shapiro, Ph.D., an assistant professor of chemical engineering at Caltech, who was not involved in the study. “The authors take an innovative approach to this problem by computationally designing antimicrobial peptides using an 'in silico' evolutionary algorithm, which scores new peptides based on a set of properties known to be correlated with effectiveness. They also include an impressive array of experiments to show that the resulting peptides indeed have the properties needed to serve as antibiotics, and that they work in at least one mouse model of infections.”

Dr. de la Fuente-Núñez and his colleagues now plan to further develop guavanin 2 for potential human use, and they also plan to use their algorithm to seek other potent antimicrobial peptides. There are currently no artificial antimicrobial peptides approved for use in human patients.

“A report commissioned by the British government estimates that antibiotic-resistant bacteria will kill 10 million people per year by the year 2050, so coming up with new methods to generate antimicrobials is of huge interest, both from a scientific perspective and also from a global health perspective,” Dr. de la Fuente-Núñez says.

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