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December 1, 2013 (Vol. 33 , No. 21)

RNA Methylation Analysis

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Zymo Research

The EZ RNA Methylation Kit is designed to facilitate bisulfite conversion of RNA for 5-methylcytosine detection. With this kit, RNA denaturation and bisulfite conversion processes are combined into a single step, and no buffer preparation is necessary. The kit includes a ready-to-use RNA conversion reagent that can be simply added to an RNA sample and incubated. Also, in-column desulfonation technology can remove precipitation steps, helping researchers obtain consistent results. The product has been designed to decrease template degradation and loss of RNA during treatment and cleanup, and to provide conversion of cytosine for methylation analysis. Recovered RNA is suitable for RT-PCR, sequencing, library preparation, and next-gen sequencing.

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