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February 15, 2015 (Vol. 35 , No. 4)

Protein Stability Measurement Instrument

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NanoTemper Technologies

The Prometheus NT.48 offers nanoDSF technology for ultra-high resolution protein stability measurements. Using intrinsic tryptophan fluorescence, nanoDSF can eliminate the need for reporter dyes in protein stability measurements. Now thermal and chemical unfolding measurements can be performed in any buffer, including detergents. The sensitivity and resolution allows for increased detection of multidomain proteins such as antibodies. With just 10 µL of reaction volume, you can measure solutions up to 150 mg/mL and down to 5 µg/mL. Applications include antibody engineering, membrane protein research, formulation, and quality control. The integrated software makes it simple to determine all the melting points of a protein or complex. The maintenance-free platform provides ease of use.

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