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April 15, 2012 (Vol. 32 , No. 8)

Protein Analysis Biocolumns

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Agilent Technologies

High-definition Zorbax 300SB-C3 and 300-Diphenyl 1.8 µm columns extend reversed-phase biomolecule separations to ultra-high-performance liquid chromatography. The C3 phase broadens the selectivity and enhances peak shape and recovery options for larger proteins including antibodies. The diphenyl phase offers additional selectivity through pi-pi interactions with aromatic amino acids in the primary structure. Additionally, the columns are stable up to 1,200 bar. StableBond technology for the C18, C8, and C3 columns, and the end-capped diphenyl with Pursuit chemistry, are designed to produce symmetrical peaks—even at low pH—when using trifluoroacetic or formic acid mobile-phase modifiers.

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