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August 1, 2012 (Vol. 32 , No. 14)

PCR Purification

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Diffinity Genomics

The RapidTip®2 can enable PCR purification with polymerase removal in just one minute with one mixing step using only a standard pipettor; no additional reagents, consumables, or equipment is needed. The technology comes prepacked in a single pipette tip. Unincorporated dNTPs, primers, primer-dimers, and DNA polymerase are removed by aspirating the PCR sample into the functional pipette tip. The purified PCR product is dispensed from the tip and may be used in downstream applications such as restriction digestion, TA cloning, or Sanger sequencing.

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