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January 15, 2013 (Vol. 33 , No. 2)

NGS Fragment Library Prep

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Beckman Coulter

SPRIselect utilizes SPRI (Solid Phase Reversible Immobilization)-based chemistry to simplify genomic DNA size selection for next-generation sequencing fragment library preparation. Following shearing, the library construction process requires size selection to produce uniform distribution of fragments. SPRIselect allows size distribution to be adjusted between 150 and 800 base pairs to suit the application and sequencing platform. The process can be performed manually or automated for high throughput in 96-well plates. SPRIselect reagent kits are available in 5, 60, and 450 mL volumes and come with guidelines to assist users in customizing protocols.

  • Beckman Coulter
  • 4300 N Harbor Blvd
  • PO Box 3100
  • Fullerton CA, 92834-3100

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