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January 1, 2012 (Vol. 32 , No. 1)

Neural Cell Transfection

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The 4D-Nucleofector™ Y unit is a new module for the 4D-Nucleofector system. The Y unit enables adherent Nucleofection of neural cells grown in 24-well tissue culture plates. For transfection, disposable conductive polymer 24-well dipping electrode arrays are inserted into the culture plate. Adherent Nucleofection overcomes a general obstacle of electroporation-based methods, i.e., the requirement for cells to be in suspension for transfection. Primary neuronal cells which typically grow in adherence in cell culture can now be kept in their physiological state and transfected by Nucleofection at a later developmental stage. This new feature also facilitates the use of the Nucleofector technology for cryopreserved Clonetics primary neurons, which do not always tolerate being transfected immediately after thawing.

  • Lonza
  • Muenchensteinerstrasse 38
  • Basel , CH-4002

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