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January 1, 2014 (Vol. 34 , No. 1)

Human NSC Immunocytochemistry Kit

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Life Technologies

An immunocytochemistry kit is available for the analysis of four common markers of human neural stem cells (NSCs)—nestin, PAX6, SOX1, and SOX2. The kit contains a set of primary and secondary antibodies, a nuclear DNA stain, and buffers for the staining protocol. The antibody combinations can allow for simultaneous assessment of two markers at a time along with nuclear DNA staining, and the dyes can enable the acquisition of multiplexed images of NSC marker expression using commonly available blue, green, and orange/red filter sets. The kit comes with reagents sufficient to run 20 samples using a 50 µL staining volume.

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