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February 1, 2012 (Vol. 32 , No. 3)

High-Resolution Melting

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Bio-Rad Laboratories

The Precision Melt Supermix delivers high-resolution melt (HRM) performance for detection of single nucleotide polymorphisms (SNP) and CpG methylation for epigenetic studies. The PCR reagent is compatible with all HRM-capable thermal cyclers. By employing the saturating EvaGreen dye, which is required for detection of class III/IV SNPs, the supermix discriminates all four SNP classes across a range of amplicons. This allows for any genomic region to be studied, regardless of amplicon length and GC content. The supermix is also fully compatible with bisulfite-converted DNA and allows for detection of CpG methylation status.

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