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July 1, 2011 (Vol. 31 , No. 13)

Gene Target Identification

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The Mission Target ID Library enables benchtop transcriptome-wide human miRNA and ncRNA gene target identification. The Target ID Library is a transcriptome-wide cDNA pool; the library’s plasmids each have a human cDNA inserted into the 3´-UTR region of a thymidine kinase-zeocin fusion protein (Tkzeo). Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, after introducing a miRNA or ncRNA of interest, for target cDNAs. Selected cDNAs are identified by sequencing. The library is provided in ready-to-use DNA format, with enough material for approximately 6 to 10 genome-wide screens.

  • Sigma-Aldrich
  • 3050 Spruce St.
  • St. Louis MO, 63103

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