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April 1, 2014 (Vol. 34 , No. 7)

Enzyme for Studying Glycosylation

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New England BioLabs

PNGase F, Recombinant is an endoglycosidase; that is, it cleaves entire carbohydrate groups from proteins. Capable of removing almost all N-linked oligosaccharides from glycoproteins, the enzyme can be used to study glycosylation. Specifically, PNGaseF, Recombinant is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides. PNGaseF digestion deaminates the asparagine residue to aspartic acid and leaves the oligosaccharide intact for further analysis. Recombinant PNGaseF is cloned from Elizabethkingia miricola (formerly Flavobacterium meningosepticum) and expressed in Escherichia coli. Reagents supplied with the product include a glycoprotein denaturation buffer, a G7 reaction buffer, and NP-40.

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