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March 1, 2012 (Vol. 32 , No. 5)

Autoinduction Media

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ZYP-5052 autoinduction media helps simplify culture handling by utilizing differential metabolism of sugars by E. coli to automatically induce protein expression. The media is packaged as a ready-to-use solution. ZYP-5052 autoinduction media activates recombinant protein expression in E. coli through diauxic growth in media containing glucose, glycerol, and lactose. In the first growth phase, bacteria preferentially metabolize a limited glucose supply to produce a high-density culture without inducing protein expression. During the second phase, bacteria consume glycerol and lactose. The presence of lactose activates lac promoters to induce expression of T7 RNA polymerase. The T7 RNA polymerase then actively transcribes the cloned DNA, which is subsequently translated into a significant percentage of the cells’ total protein. The recombinant protein may then be isolated and purified for downstream applications.

  • Amresco
  • 6681 Cochran
  • Solon OH, 44139-0098

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