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Glycosylation profoundly affects the efficacy and safety of therapeutic proteins, influencing their biological activity, serum half-life, and immunogenicity. This post-translational modification, determined to a large degree by choice of expression systems, particularly impacts the function of antibodies and other therapeutic proteins with essential N-glycosylation sites.

The critical nature of N-glycosylation requires that protein analytical scientists in the biopharmaceutical industry characterize and monitor glycosylation regardless of whether they are working on innovator, biosimilar, or biobetter therapeutic molecules.

While N-glycan analysis remains challenging and time-consuming, hydrophilic interaction chromatography (HILIC) of released, fluorescently labeled N-glycans offers a powerful, robust, and scalable approach to glycosylation analysis applicable in the research and development lab through to quality control.

Webinar panelists will discuss critical steps in achieving efficient workflow emphasizing sample prep and separation optimization as key elements for successful N-glycan analysis. Oscar Potter, Ph.D., research scientist, will lead the discussion on the workflow for relative quantitation of N-glycans using a novel HILIC column with UHPLC/HPLC and FLD. James Martosella, applications scientist, will review the steps of the glycan prep for this analysis. In addition, Sonja Schneider, applications scientist, will show how to interpret the fluorescence chromatogram and assign N-glycan structures through the use of online mass spectrometry with a QTOF. Finally, Aled Jones will provide information about the use of a modular platform that streamlines glycoprotein sample preparation, enabling purification, glycan release, and labeling.

Who Should Attend

  • Protein biochemists
  • QC/QA scientists manufacturing therapeutic proteins
  • Protein analytical scientists interested in glycan analysis
  • Therapeutic protein developers

You Will Learn

  • How to implement workflow solutions for fast analysis of glycans from glycan prep to analysis using LC/FLD
  • How to obtain N-glycan structure confirmation using online mass spectrometry
  • How time-consuming glycan prep may be automated for high-throughput results
  • How characterization of N-glycan profiles can be achieved throughout various phases of mAb development
  • How the GlykoPrep® modular platform dramatically streamlines N-glycan sample preparation, enabling rapid glycan release and labeling for analysis in hours, rather than days

Produced with support from:

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Oscar Potter, Ph.D.
Research Scientist,
Agilent Labs

Sonja Schneider, Ph.D.
Application Scientist for Bio-Related Analysis,
Agilent Technologies

James Martosella
Applications Scientist,

Aled Jones, Ph.D.
Technical Liaison,