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Peptide mapping is a critical quality test to confirm the primary structure of proteins used in biotherapeutics such as monoclonal antibodies and antibody-drug conjugates. It relies on protein digestion which includes the enzymatic treatment of a protein to produce peptide fragments to further characterize specific regions and modifications. The results of protein digests are essential for protein structural characterization, protein identification, and protein modification monitoring including post-translation modifications, product related impurities, and multi-attribute monitoring (MAM) methods. However, sample preparation methods for peptide mapping are often complex, laborious, irreproducible, and require long digestion times—as much as 24 hours in some cases.

In this GEN webinar, Dr. Caitlin Hanna describes an automatable sample preparation protocol that simplifies peptide mapping and reduces long digestion times in both regulated and research applications. She highlights the key challenges of preparing samples for peptide mapping and shares strategies for achieving reproducible protein digests using RapiZyme™ Trypsin and the PeptideWorks™ Tryptic Protein Digestion Kit for sample prep.

A live Q&A session followed the presentation, offering a chance to pose questions to our expert panelist.

Caitlin Hanna
Caitlin Hanna, PhD
Senior Scientist