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During the activation of humoral immune responses, B cells acquire antigen for subsequent presentation to cognate T cells. Imaging flow cytometry reveals that antigen polarization is preserved during B cell division, promoting asymmetric antigen segregation among progeny. The generation of progeny with differential capacities for antigen presentation may have implications for somatic hypermutation and class switching during affinity maturation and as B cells commit to effector cell fates.

Rigorous methods for the detection of DNA-damaged foci in eukaryotic cells are fundamental to DNA repair studies, which delineate mechanisms and may identify differences in DNA repair capacity among cell types. The Parris lab performed the first analyses to demonstrate the use of imaging flow cytometry for the detection of γ-H2AX foci in cells exposed to ionizing radiation that induces DNA double strand breaks. This webinar presents data validating the enhancement of foci quantitation and image resolution employing imaging flow cytometry, using cell lines derived from normal individuals.

In this webinar, Dr. Thaunat’s work demonstrates that imaging flow cytometry (IFC) is key to the identification of polarized distribution of antigen on B cells that persists in vivo.  Dr. Parris will describe how IFC with multimagnification and extended depth of field enhances quantitation of DNA damage in cells, providing a quantitative alternative to traditional low-throughput in situ microscopy methods for the detection of γ-H2AX foci.

Who Should Attend:

  • Immunologists
  • Flow cytometrists
  • Toxicologists
  • Drug development scientists
  • Cancer biologists

You Will Learn

  • How imaging flow cytometry is used to detect and quantitate the aggregation of antigen on antigen-presenting cells
  • Methods for quantitation of γ-H2AX foci for precise assessment of DNA damage in large populations, key to evaluating the effects of radiation and chemotherapeutics, and for novel compound screening

Produced with support from:

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Olivier Thaunat, M.D.
Assistant Professor,
University of Lyon

Christopher Parris, Ph.D.
Director, Division of Biosciences—Life Sciences,
Brunel University London, College of Health and Life Sciences