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Identifying conditions that maximize the structural stability of a biologic’s native state is essential for developing therapeutics, formulations, and process development conditions. In this GEN webinar our panelists will present methods and results using chemical denaturation as a way to determine the Gibbs free energy (ΔG) of proteins in a given formulation. The HUNK, a fully automated chemical denaturation platform, provides quantitative data on protein stability, allowing researchers to rank protein constructs, screen and optimize formulations, and assess the propensity for denaturation and aggregation. Traditionally, aggregation is measured over long periods of time using size exclusion chromatography. By increasing protein concentration and measuring the change in ΔG (ΔGtrend), the HUNK can measure aggregation immediately and distinguish native from denatured state aggregation.

Our first presentation will be given by Joe Barco, Ph.D., Senior Product Manager at Unchained Labs. Dr. Barco will provide a brief introduction to the HUNK, a fully automated chemical denaturation platform.

The second presentation will be provided by Katherine Bowers, Ph.D., Principal Scientist at Fujifilm Diosynth Biotechnologies. Dr. Bowers will present chemical denaturation data and its application to stability determination for protein comparability, formulation optimization and downstream process development.

Who Should Attend

  • Researchers interested in avoiding protein aggregations
  • Scientists involved in biophysical protein characterizations
  • Researchers interested in protein stability
  • Scientists involved in formulation screening and optimization

You Will Learn

  • The use of chemical denaturation for measuring biologic stability (ΔG).
  • The application of the HUNK platform for routine determination of stability (ΔG) in different formulations and for protein comparability.
  • How to immediately determine protein aggregation states (ΔGtrend).

Produced with support from:

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Joseph Barco, Ph.D.
Senior Product Manager,
Unchained Labs

Katherine Bowers, Ph.D.
Principal Scientist,
Fujifilm Diosynth Biotechnologies